Publicaties

2025

Aberrant strand transfer events across multiple stages of reverse transcription shape the heterogeneous landscape of the HIV-1 reservoir

Communications Biology volume 8, Article number: 1703 (2025)

Jarryt Hardy, Virginie Mortier, Yiannis De Meersman, Delfien Staelens, Els Demecheleer, Marlies Schauvliege, Ciel Vermeersch, Elizaveta Padalko and Chris Verhofstede

2024

Reverse transcription of plasma-derived HIV-1 RNA generates multiple artifacts through tRNA(Lys-3)-priming

Microbiology Spectrum, Immunology, Research Article, 5 March 2024

Jarryt Hardy, Els Demecheleer, Marlies Schauvliege, Delfien Staelens, Virginie Mortier and Chris Verhofstede

Longitudinal patterns of inflammatory mediators after acute HIV infection correlate to intact and total reservoir

Front. Immunol., 05 January 2024 Sec. Viral Immunology Volume 14 - 2023

Jozefien De Clercq, Marie-Angelique De Scheerder, Virginie Mortier, Chris Verhofstede, Stefaan J. Vandecasteele, Sabine D. Allard, Coca Necsoi, Stephane De Wit, Sarah Gerlo, Linos Vandekerckhove

2023

Towards Novel HIV-1 Serodiagnostic Tests without Vaccine-Induced Seroreactivity

ASM Journals Microbiology Spectrum Vol. 11, No. 3

Ole Lagatie, Dax Lauwers, Harvinder Singh, Fien Vanroye, Daniel J. Stieh, Johan Vingerhoets, Ludo Lavreys, Valérie Oriol-Mathieu, Will Colón, Chris Verhofstede, Koen Vercauteren, Dorien Van den Bossche, Maria Grazia Pau

2022

Impact of COVID-19 on the Belgian HIV epidemic: slowdown of HIV transmission and testing and adaptation of care.

BMC Infectious Diseases, volume 22, Article number 901 (2022)

Van Beckhoven D, Serrien B, Montourcy M, Verhofstede C, Van den Bossche D, Libois A, De Geyter D, Martin T, Van den Eynde S, Vuylsteke B, Darcis G, van Halem K, Florence E, Deblonde J.

Profile and prevention trajectory of recently HIV infected patients in Belgium.

Journal of the International AIDS Society 2022 (25). Meeting Abstract: P019

Vanden Bulcke C, Deblonde J, Callens S, Verhofstede C.

Prevalence and evolution of transmitted HIV drug resistance in Belgium between 2013 and 2019

Open Forum Infectious Diseases, ofac195

Virginie Mortier, Laurent Debaisieux, Géraldine Dessilly, Karolien Stoffels, Dolores Vaira, Ellen Vancutsem, Kristel Van Laethem, Fien Vanroye, Chris Verhofstede

2021

Screening algorithms for HBV, HCV, HIV and syphilis in an anatomical donation program

Annals of Anatomy - Anatomischer Anzeiger Volume 239, January 2022, 151805

Brecht Valcke, Koen Vercauteren, Elizaveta Padalko, Chris Verhofstede, Katharina D’Herde, Wouter Willaert

2020

Benefits of antiretroviral therapy initiation during acute HIV infection

Acta Clinica Belgica, International Journal of Clinical and Laboratory Medicine Volume 77, 2022 - Issue 1

Jozefien De Clercq, Sofie Rutsaert, Marie-Angélique De Scheerder, Chris Verhofstede, Steven Callens, Linos Vandekerckhove

Chronic and Early Antiretroviral Therapy Impact Human Immunodeficiency Virus (HIV) Serological Assay Sensitivity, Leading to More False-Negative Test Results in HIV Diagnosis

The Journal of Infectious Diseases, Volume 222, Issue 10, 15 November 2020, Pages 1660–1669

Karolien Stoffels, Fien Vanroye, Virginie Mortier, Laurent Debaisieux, Marie-Luce Delforge, Melissa Depypere, Géraldine Dessilly, Dolores Vaira, Ellen Vancutsem, Sigi Van den Wijngaert, Kristel Van Laethem, Koen O A Vercauteren, Chris Verhofstede, Katrien Fransen

High frequency of new recombinant forms in HIV-1 transmission networks demonstrated by full genome sequencing

Infection, Genetics and Evolution, Volume 84, October 2020, 104365

Laura Hebberecht, Virginie Mortier, Kenny Dauwe, Marlies Schauvliege, Delfien Staelens, Els Demecheleer, Karolien Stoffels, Fien Vanroye, Marie-Luce Delforge, Ellen Vancutsem, Géraldine Dessilly, Dolorès Vaira, Kristel Van Laethem, Chris Verhofstede

2019

Single genome sequencing of near full-length HIV-1 RNA using a limiting dilution approach

Journal of Virological Methods, Volume 274, December 2019

Laura Hebberecht, Leen Vancoillie, Marlies Schauvliege, Delfien Staelens, Els Demecheleer, Jarryt Hardy, Virginie Mortier, Chris Verhofstede

Exploring HIV-1 Transmission Dynamics by Combining Phylogenetic Analysis and Infection Timing

Viruses 2019, 11(12), 1096

Chris Verhofstede, Virginie Mortier , Kenny Dauwe , Steven Callens , Jessika Deblonde , Géraldine Dessilly , Marie-Luce Delforge , Katrien Fransen , André Sasse , Karolien Stoffels , Dominique Van Beckhoven , Fien Vanroye , Dolores Vaira , Ellen Vancutsem and Kristel Van Laethem

2018

Quantification of total HIV-1 DNA in buffy coat cells, feasibility and potential added value for clinical follow-up of HIV-1 infected patients on ART

Journal of Clinical Virology, Volume 106, September 2018, Pages 58-63

Virginie Mortier, Els Demecheleer, Delfien Staelens, Marlies Schauvliege, Kenny Dauwe, Laura Hebberecht, Leen Vancoillie, Chris Verhofstede

Frequency of occurrence of HIV-1 dual infection in a Belgian MSM population.

PLoS One. 2018 Apr 6;13(4).

Hebberecht Laura; Vancoillie Leen; Schauvliege Marlies; Staelens Delfien; Dauwe Kenny; Mortier Virginie; Verhofstede Chris.

Phylogenetic analysis of the Belgian HIV-1 epidemic reveals that local transmission is almost exclusively driven by men having sex with men despite presence of large African migrant communities.

Infection, Genetics and Evolution, 2018, Mar6; 61:36-44.

Chris Verhofstede; Kenny Dauwe; Katrien Fransen; Kristel Van Laethem; Sigi Van den Wijngaert; Jean Ruelle; Marie-Luce Delforge; Ellen Vancutsem; Dolores Vaira; Karolien Stoffels; Sergio Garcia Ribas; Géraldine Dessilly; Laurent Debaisieux; Denis Pierard; Marc Van Ranst; Marie-Pierre Hayette; Jessica Deblonde; Andre Sasse; Dominique Van Beckhoven; Virginie Mortier.

2017

Decision tree for accurate infection timing in individuals newly diagnosed with HIV-1 infection.

BMC infectious diseases 2017, 17: 738.

Chris Verhofstede; Katrien Fransen; Annelies Van Den Heuvel; Kristel Van Laethem; Jean Ruelle; Ellen Vancutsem; Karolien Stoffels; Sigi Van den Wijngaert; Marie-Luce Delforge; Dolores Vaira; Laura Hebberecht; Marlies Schauvliege; Virginie Mortier; Kenny Dauwe; Steven Callens.

Meticulous plasma isolation is essential to avoid false low-level viraemia in Roche Cobas HIV-1 viral load assays.

Antiviral Therapy, 2017 Oct 24.

Virginie Mortier; Leen Vancoillie; Kenny Dauwe; Delfien Staelens; Els Demecheleer; Marlies Schauvliege; Sylvie Dinakis; Tom Van Maerken; Géraldine Dessilly; Jean Ruelle; Chris Verhofstede.

Longitudinal sequencing of HIV-1 infected patients with low-level viremia for years while on ART shows no indications for genetic evolution of the virus.

Virology 2017, 510: 185-193.

Leen Vancoillie; Laura Hebberecht; Kenny Dauwe; Els Demecheleer; Sylvie Dinakis; Dries Vaneechoutte; Virginie Mortier; Chris Verhofstede.

2016

Deep Sequencing of HIV-1 RNA and DNA in Newly Diagnosed Patients with Baseline Drug Resistance Showed No Indications for Hidden Resistance and Is Biased by Strong Interference of Hypermutation.

J Clin Microbiol. 2016 Jun;54(6):1605-15

Dauwe K, Staelens D, Vancoillie L, Mortier V, Verhofstede C.

Late presentation to HIV testing is overestimated when based on the consensus definition.

HIV Med 17(3): 231-234

Sasse, A., E. Florence, A. Pharris, S. De Wit, P. Lacor, D. Van Beckhoven, J. Deblonde, M. L. Delforge, K. Fransen, J. C. Goffard, J. C. Legrand, M. Moutschen, D. Pierard, J. Ruelle, D. Vaira, B. Vandercam, M. Van Ranst, E. Van Wijngaerden, L. Vandekerckhove, C. Verhofstede

2015

HIV-1 RNA and HIV-1 DNA persistence during suppressive ART with PI-based or nevirapine-based regimens.

J Antimicrob Chemother 70(12): 3311-3316

Kiselinova, M., A. M. Geretti, E. Malatinkova, K. Vervisch, A. Beloukas, P. Messiaen, P. Bonczkowski, W. Trypsteen, S. Callens, C. Verhofstede, W. De Spiegelaere, L. Vandekerckhove

Characteristics and spread to the native population of HIV-1 non-B subtypes in two European countries with high migration rate.

BMC Infectious Diseases.2015, 15:524

Dauwe Kenny, Mortier Virginie, Schauvliege Marlies, Van Den Heuvel Annelies, Fransen Katrien, Servais Jean-Yves, Bercoff Danielle, Seguin-Devaux Carole, Verhofstede Chris.

Good continuum of HIV care in Belgium despite weaknesses in retention and linkage to care among migrants.

BMC Infect Dis 15: 496

Van Beckhoven, D., E. Florence, J. Ruelle, J. Deblonde, C. Verhofstede, S. Callens, E. Vancutsem, P. Lacor, R. Demeester, J. C. Goffard, A. Sasse

Impact of a decade of successful antiretroviral therapy initiated at HIV-1 seroconversion on blood and rectal reservoirs.

Elife 4: e09115

Malatinkova, E., W. De Spiegelaere, P. Bonczkowski, M. Kiselinova, K. Vervisch, W. Trypsteen, M. Johnson, C. Verhofstede, D. de Looze, C. Murray, S. Kinloch-de Loes, L. Vandekerckhove

Drug resistance is rarely the cause or consequence of long-term persistent low-level viraemia in HIV-1-infected patients on ART.

Antivir Ther 20(8): 789-794

Vancoillie, L., V. Mortier, E. Demecheleer, M. Schauvliege, L. Vandekerckhove, D. Vogelaers, C. Verhofstede

Comparison of methods for in-house screening of HLA-B*57:01 to prevent abacavir hypersensitivity in HIV-1 care.

PLoS One 10(4): e0123525

De Spiegelaere, W., J. Philippe, K. Vervisch, C. Verhofstede, E. Malatinkova, M. Kiselinova, W. Trypsteen, P. Bonczkowski, D. Vogelaers, S. Callens, J. Ruelle, K. Kabeya, S. De Wit, P. Van Acker, V. Van Sandt, M. P. Emonds, P. Coucke, E. Sermijn, L. Vandekerckhove

2014

Determinants of adherence in a cohort of Belgian HIV patients: a pilot study.

Acta Clinica Belgica 69 (2): 111-115.

Degroote S., Vogelaers D., Vermeir P., Mariman A., De Rick A., Van Der Gucht B., Pelgrom J., Van Wanzeele F., Verhofstede C., Vancauwenberghe J. and Vandijck D.

Antiretrovirals for HIV prevention: when should they be recommended?

Expert Rev. Anti Infect. Ther. 12: 431-445.

Kiselinova M., De Spiegelaere W., Verhofstede C., Callens S. and Vandekerckhove L.

Markers associated with persisting low-level viraemia under antiretroviral therapy in HIV-1 infection.

Journal of Antimicrobial Chemotherapy 69: 1098-1103.

Vancoillie L., Demecheleer E., Callens S., Vogelaers D., Vandekerckhove L., Mortier V. and Verhofstede C.

Comparative Performances of HIV-1 RNA Load Assays at Low Viral Load Levels: Results of an International Collaboration.

Journal of Clinical Microbiology 52: 517-523.

Swenson L. C., Cobb B., Geretti A. M., Harrigan P. R., Poljak M., Seguin-Devaux C., Verhofstede C., Wirden M., Amendola A., Boni J., Bourlet T., Huder J.B., Karasi J.C., Lepej S.Z., Lunar M.M., Mukabayire O., Schuurman R., Tomazic J., Van Laethem K., Vandekerckhove L. and Wensing A.M.J.